Technical Data
L1669-06A
Leptin (Ob, Obese Protein, Obesity Factor) (Biotin)
Description:
Application(s):
ELISA (direct and indirect; 1:5000-1:10000)
Western Blot (1:5000-1:10000 using ECL; suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
1mg/ml
Immunogen:
Recombinant mouse leptin.
Purity:
Purified
Form
Liquid. Purified antibody in PBS, pH 7.4. Contains no preservatives.
Specificity:
Recognizes human (weak), mouse and rat (weak) leptin. Detects a band of ~16kDa by Western blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.