		Technical Data
L1669-07	Leptin (Ob, Obese Protein, Obesity Factor)

Description:
Applications:
Suitable for use in ELISA, Western Blotting, Other applications not tested.

Recommended Dilution:
ELISA (direct and indirect): 1:5000-1:10,000).
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at RT).
Optimal conditions must be determined individually for each application.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6D524

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Mouse

Concentration:

Immunogen:

Recombinant human leptin:Fc expressed from mammalian cells.

Purity:

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

Specificity:

Recognizes both natural and recombinant human leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with mouse or rat leptin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.