Technical Data
L1669-07
Leptin (Ob, Obese Protein, Obesity Factor)
Description:
Applications:
Suitable for use in ELISA, Western Blotting, Other applications not tested.

Recommended Dilution:
ELISA (direct and indirect): 1:5000-1:10,000).
Western Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at RT).
Optimal conditions must be determined individually for each application.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D524
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
Immunogen:
Recombinant human leptin:Fc expressed from mammalian cells.
Purity:
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:
Recognizes both natural and recombinant human leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with mouse or rat leptin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.