Technical Data
L1670-10H
Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, FLJ94114, Ob, Obese Protein, Obesity Factor, Obesity Homolog Mouse, OBS)
Description:
Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acide residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
L1670-17G: Leptin, Recombinant, Human

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
E. coli-expressed recombinant human leptin
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human Leptin. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.