		Technical Data
L1670-12	Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)

Description:
Leptin (from the Greek leptos, meaning thin) is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately 16kD in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure.

Cellular Localization: Secreted

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8L577

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice		Mouse

Concentration:

As reported

Immunogen:

Recombinant full length protein.

Purity:

As reported

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

This antibody recognizes recombinant human leptin and leptin circulating in human blood. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species, but may be crossreactive to mouse and rat due to 85% and 84% homology to human leptin respectively.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Rahmouni K & Haynes WG Leptin and the cardiovascular system. Recent Prog Horm Res 59:225-44 (2004). El-Atat F et al. Obesity and hypertension. Endocrinol Metab Clin North Am 32:823-54 (2003). Zhang Y et al. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-32 (1994).