Technical Data
L1670-12A
Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)
Description:
Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.

Cellular Localization: Secreted

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18L578
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant full length protein (Human).
Purity:
As reported
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
This antibody recognizes recombinant human leptin and leptin circulating in human blood. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species, but may crossreact with mouse and rat due to 85% and 84% sequence homology respectively.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Rahmouni K & Haynes WG Leptin and the cardiovascular system. Recent Prog Horm Res 59:225-44 (2004). El-Atat F et al. Obesity and hypertension. Endocrinol Metab Clin North Am 32:823-54 (2003). Zhang Y et al. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-32 (1994).