Technical Data
L1670-12J
Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)
Description:
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:4000. Detects a ~16kD protein representing leptin from 3T3-L1 cell extract.
Immunohistochemistry (Frozen): 1:1000.
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide correspoding to amino acids 25-44 [Q(25)KVQDDTKTLIKTIVTRIND(44)] of mouse leptin. This sequence is completely conserved between mouse and bovine. Cellular Localization: Secreted
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1 mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes mouse Leptin. Species Crossreactivity: human, rat, and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Li HY et al. Leptin immunoreactivity in the central nervous system in normal and diabetic rats. Neuroreport 10:437-42 (1999).