		Technical Data
L1670-12L	Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)

Description:
Regulation of food intake and energy expenditure by leptin is thought to predominantly occur in the hypothalamus of the brain, where leptin binds with high affinity to neuronal leptin receptors (OB-R), inhibiting the action of the feeding stimulants neuropeptide Y (NPY) and agouti-related peptide (AgRP), or promoting the effects of the appetite regulator a-melanocyte-stimulating hormone (a-MSH). Leptin also acts as a direct stimulator of fatty acid oxidation in the mitochondria of liver and skeletal muscle cells, and influences glucose and fat metabolism. The expression of leptin in relation to metabolic status is influenced by hormonal and metabolite control: stimulators of leptin include insulin and cortisol, whilst cAMP, thiazolidinediones and beta-adrenergic receptor agonists, reduce leptin expression.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Sheep

Concentration:

~5mg/ml

Immunogen:

HMW conjugated leptin amino acids 57-70.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes leptin, a 16kDa secreted protein hormone encoded by the OB gene, which plays a key role in the regulation of metabolism, body weight and reproduction and is expressed primarily by white adipocytes, and to a lesser extent by stomach endothelium and the placenta. Species crossreactivity: human, mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mantzoros, C.S. (1999) The role of leptin in human obesity and disease: A review of current evidence. Ann. Intern. Med. 130: 671-680.2. Houseknecht, K.L. et al. (1998) The biology of leptin: A review. J. Anim. Sci. 76: 1405-1420.