Technical Data
Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 147 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence the reproductive function.

Suitable for use in ELISA and Western blotting.

Titer: Defined by indirect ELISA is >1:100,000 for antibody concentration 1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.

Reconstitution: Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of
the antibody. In this case clarify the solution by centrifugation.

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Recombinant mouse leptin.
Immunoaffinity chromatography on a column with immobilized mouse leptin.
Sterile filtered and lyophilized from 1mg/ml 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.
The recombinant mouse leptin is 100% homologous with the mouse serum leptin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang Y., Proenca R., Maffei M., Barone M., Leopold L., Friedman J.: Positional Cloning of the Mouse Obese Gene and its Human Homologue. Nature 372, 425-432 (1994)

2. Halaas J.L., Gajiwala K.S., Maffei M. et al.: Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543-546 (1995)

3. Maffei M., Halaas J., Ravussin E. et al.: Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1, 1155-1161 (1995)

4. Lonnqvist F., Arner P., Nordfors L. and Shalling M.: Overexpression of the obese (ob) gene in adipose tissue of human subjects. Nature Med. 1, 950-953 (1995)