Technical Data
L1671-03A
Leptin Receptor (OBR, LEPR, B219, Obese Receptor)
Description:
Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16kD protein consisting of 146aa residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816aa residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15i316Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa1-839 from human Leptin Receptor expressed in NS0 mouse myeloma cell line.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophillized powder from 0.05M PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human Leptin Receptor. Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Campfield, L.A., Smith, F.J. and Burn, P., Horm. Metab. Res. 28: 619-632 (1996). 2. Cioffi, J.A., et al., Nat. Med. 2(5): 585-589 (1996). 3. Tartagalia, L.A., Dembski, M., et al., Cell 83: 1263-1271 (1995).

Wang Y., Kuropatwinski K.K. et al.: Leptin receptor action in hepatic cells. J. Biol. Chem.