Technical Data
Leucine Rich Repeat Containing 1 (Leucine-rich Repeat-containing Protein 1, LRRC1, LANO Adapter Protein, LAP and no PDZ Protein, LANO)
Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Whole cell lysate from PC-3 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to human Lano.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human Lano.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5. 2. Legouis R, Jaulin-Bastard F, Schott S, et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 2003; 4:1096-102. 3. Funke L, Dakoji S, Bredt DS. Membrane-Associated Guanylate Kinases Regulate Adhesion and Plasticity at Cell Junctions. Annu. Rev. Biochem. 2005; 74:219-45. 4. Bilder D and Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000; 403:676-80. 5. Legouis R, Gansmuller A, Sookhareea S, et al. LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans. Nat. Cell Biol. 2000; 2:415-22.