Technical Data
L2040-11A
Leukemia Inhibitory Factor, Recombinant, Human (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

Recombinant protein corresponding to aa23-202 of human LIF protein, fused to His GST-tag at N-terminus, expressed in E.coli.

AA Sequence:
MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF

Molecular Weight: 47.2kD (415aa) confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
47.2kD
Source: E.coli
Purity: ~90% (SDS-PAGE).
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol, 50mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Gillett N A., et al. (1993) Growth Factors. 9:301-305. 2. Stahl N., et al. (1994) J Neurobiol. 25:1454-1466.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.