Technical Data
L2046-01D
Leukotriene B4 Receptor (BLTR) (PE)
Description:
BLT1 has been reported in blood, bone marrow, brain, heart, liver, and skeletal muscle. In addition, BLT1 has been shown to be highly expressed in leukocytes and lymphoid tissues such as spleen, thymus, and lymph node. ESTs have been isolated from B-cell/lung/testis, breast, colon, eye, kidney, skin, spleen, testis, and tonsil libraries.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:2. Use 10ul to label 10e6 cells or 100ul whole blood.
Optimal dilution determined by the researcher

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Recommended Negative Controls:
I1904-84B2: IgG2a Negative Control, Murine (PE)

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6A429Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
HeLa cell transfectants expressing human BLTR.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyphilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes the human BLTR molecule, the leukotriene B4 receptor. BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pettersson, A., et al., Biochem. Biophys. Res. Commun. 279: 520-525 (2000). 2. Costa, M.F., et al., J. Leukoc. Biol. 87: 323332 (2010). 3. Islam, S.A., et al., Blood 107: 444453 (2006). 4. Medoff, B.D., et al., J. Exp. Med. 202: 97110 (2005). 5. Serezani, C.H., et al., Blood 114: 33163324 (2009). 6. Heller, E.A., et al., Circulation 112: 578586 (2005). 7. Thomas, S.Y., et al., J. Immunol. 179: 19011912 (2007). 8. Sabirsh, A., et al., FJ Lipid Res. 46: 13391346 (2005). 9. Loell, I., et al., Ann. Rheum. Dis. 72(2): 293-299 (2013). 10. Wang, L., et al., Am. J. Respir. Crit. Care Med. 186(10): 989-998 (2012).