Technical Data
LNX1 (Ligand of Numb-protein X 1, LNX, MPDZ, PDZRN2, Multi-PDZ-domain-containing protein)
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:16,000.
Western Blot: 0.5ug/ml. Preliminary experiments gave next to 70kD bands at approx 75kD and 27kD in Human Kidney and Lung lysates. Optimal dilutions to be determined by the reseacher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See L2192-03-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PabIgGAffinity Purified
100ug-20°CBlue IceHumanGoat
A synthetic peptide from the C-terminus of aa-PDAYRPRDDSFH
Purified by affinity chromatography.
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes human LNX1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Characterization of human LNX, a novel ligand of Numb protein X that is downregulated in human gliomas., Chen J, Xu J, Zhao W, Hu G, Cheng H, Kang Y, Xie Y, Lu Y. , Int J Biochem Cell Biol. 2005 Nov;37(11):2273-83. Epub 2005 Apr 11.