Technical Data
Lim3 (LHX3, P-Lim, Lim Region Containing Transcription Factor)
LHX3 is one of several transcription factors that have been implicated in pituitary development. LHX3 is critical for motor neuron specification and the development of the anterior and intermediate lobes of the pituitary gland in mammals. A point mutation in the LHX3 gene has recently been implicated in a severe form of combined pituitary hormone deficiency in humans. Patients who have both a missense mutation and an intragenic deletion in the LHX3 gene display retarded growth, pituitary hormone deficiency and abnormal neck and cervical spine development. Recent laboratory studies show that mutant forms of the LHX3 protein are not fully able to activate pituitary hormone gene promoters. Mice with a targeted disruption of the LHX3 gene are usually stillborn or die shortly after birth and do not possess either the anterior or intermediate lobes of the pituitary gland. The possibility exists that other mutations in the LHX3/P-LIM/LIM-3 gene may result in many additional types of pituitary/growth conditions in both humans and animals. With this new information, various assays, tests, and ultimately treatments could be developed for conditions/diseases related to mutations in the LHX3/P-LIM/LIM-3 gene.

Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation, and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Recommended fixative: MEMFA. Immunohistochemistry (Paraffin): 1:250-1:1000
Immunohistochemistry (Frozen): 1:3000-1:6000
Immunohistochemistry (Whole mounts): 1:4000-1:8000
Western Blot: 1:2500-1:5000
Immunoprecipitation: 1:200
Immunocytochemistry: 1:200-1:500
Immunofluorescence: Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceMouseRabbit
C-terminal portion of mouse Lim3.
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Recognizes mouse Lim3. Does not appear to crossreact with gsh-4 on tissue sections, but may crossreact in Immunoprecipitation and Western Blot. Species Crossreactivity: human, rat, frog, fish and LIM-3 protein from whole-mount fish embryo.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McGillivray, S.M., et al., Endocrinology 146: 2180-2185 (2005).