Technical Data
L2279-95
Lin28b (Protein Lin-28 Homolog B, Lin-28B, Lin-28.2)
Description:
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as ras, myc and Hmga2 (3). It has recently been shown that LIN28A and LIN28B are upregulated in primary human tumors and in human cancer cell lines, their overexpression directly correlating to downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5), and allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2 and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues near the carboxy terminus of mouse LIN28B. Species sequence homology: rat
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total mouse LIN28B protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25. (2) Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4. (3) Cho, W.C. (2007) Mol Cancer 6, 60. (4) Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8. (5) West, J.A. et al. (2009) Nature 460, 909-13. (6) Ong, K.K. et al. (2009) Nat Genet 41, 729-33. (7) Yu, J. et al. (2007) Science 318, 1917-20.