Technical Data
L2499-14M
Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9) (Biotin)
Description:
Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 (1) and subsequently identified as a lipocalin (2). Unlike human NGAL, it is an acute phase protein upregulated in the liver (3), but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury (4). It is also upregulated in the involuting uterus and mammary gland (5).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k13A135Purified
SizeStorageShippingSourceHost
50ul-20°CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Recombinant mouse NGAL adsorbed onto aluminum hydroxide gel.
Purity:
Purified
Form
Supplied as a liquid in 0.01M phosphate buffer, pH 7.4, 0.14M NaCl and 15mM sodium azide.
Specificity:
Recognizes mouse NGAL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hraba-Renevey S, Türler H, Kress M, Salomon C, Weil R (1989) SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism. Oncogene 4:601-608. 2. Flower DR, North AC, Attwood TK (1991) Mouse oncogene protein 24p3 is a member of the lipocalin protein family. Biochem Biophys Res Commun 180:69-74. 3. Liu Q, Nilsen-Hamilton M (1995) Identification of a new acute phase protein. J Biol Chem 270:22565-22570. 4. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-2543. 5. Ryon J, Bendickson L, Nilsen-Hamilton M (2002) High expression in involuting reproductive tissues of uterocalin/24p3, a lipocalin and acute phase protein. Biochem J 367:271-277.