Technical Data
L2499-17E
Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)
Description:
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25kD glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3).

Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including alpha1-microglobulin and beta-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. Human neutrophil gelatinase-associated lipocalin (NGAL), and its mouse analog 24p3, are members of the lipocalin family of small secreted proteins. These proteins are up-regulated in a number of pathological conditions, including cancers, and may function as transporters of essential factors.

Applications:
Suitable for use in ELISA, Western Blot. Not suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA (as capture antibody): 1:30,000 (recombinant human NGAL coated directly at 0.8mg/ml.). Forms a sandwich pair with L2499-16 (as biotinylated detection antibody) for measuring cynomolgus monkey (Macaca fascicularis) and binds human NGAL in solution.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k8D2Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human NGAL
Purity:
Purified by Protein A affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Binds cynomolgus monkey (Macaca fascicularis) NGAL and cross-reacts with human NGAL. Binds human NGAL in complex with MMP-9.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-2543.