Technical Data
Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay™ Kit
Kits and Assays Storage: -20°CShipping: Blue Ice
Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol plays a central role in various disease developments. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events. The HDL and LDL/VLDL Cholesterol Quantification Kit provides a simple
quantification method of HDL and LDL/VLDL after a convenient separation of HDL from LDL
and VLDL (very low-density lipoprotein) in serum samples. In the assay, cholesterol oxidase
specifically recognizes free cholesterol and produces products which react with probe to
generate color (lambda = 570nm) and fluorescence (Ex/Em = 538/587nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free
cholesterol can be detected separately in the presence and absence of cholesterol esterase
in the reactions.

Kit Components:
Cholesterol Assay Buffer 1x25ml
2X LDL/VLDL Precipitation Buffer 1x10ml
Cholesterol Probe (in DMSO, anhydrious) 1x200ul
Enzyme Mix (Lyophilized) 1x1vial
Cholesterol Esterase (Lyophilized) 1x1vial
Cholesterol Standard (2ug/ul) 1x100ul.

Storage and Stability:
Store the kit at -20°C, protect from the light. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.