Technical Data
L2601-02
Lipoprotein Receptor, Low Density (LDLR, LDL Receptor)
Description:
Low-density lipoprotein (LDL) is one of the five major groups of lipoproteins, which in order of size, largest to smallest, are chylomicrons, VLDL, IDL, LDL and HDL, that enable lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. Blood tests typically report LDL-C, the amount of cholesterol contained in LDL. In clinical context, mathematically calculated estimates of LDL-C are commonly used to estimate how much low density lipoproteins are driving progression of atherosclerosis. Direct LDL measurements are also available.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunofluorescence Microscopy. Other applications have not been tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k4C4Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceBovineMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Purified bovine adrenal cortex LDL Receptor.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes bovine adrenal cortex LDL receptor. Species Crossreactivity: human. Does not recognize rat, mouse, hamster, canine or rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beisiegel, U., et al., J. Biol. Chem. 256: 11,923-11,931 (1981). 2. Beisiegel, U., et al., J. Biol. Chem. 257: 13,150-13,156 (1982). 3. Schmitz, G., et al., Arteriosclerosis & Thrombosis 13: 1053-1065 (1993). 4. Virgolini, I., et al., Arteriosclerosis, Thrombosis & Vascular Biology 15: 17-26 (1995).