		Technical Data
L2601-02A	Lipoprotein Receptor, Low Density (LDLR, LDL Receptor) (Biotin)

Description:
The LDL receptor (160kD mature or glycosylated receptor; 120kD precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.

Application:
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry, Western Blot and Analysis of LDL receptor function in patients with familial hypercholesterolemia. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:10
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cells and liver sections

Negative Control:
NIH 3T3 cells and normal skin

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	4C5	Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Purified bovine adrenal cortex LDL Receptor

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, before the addition of glycerol to 40%. Labeled with Biotin.

Specificity:

Recognizes an epitope in the region of repeat #1 of the human ligand binding region. Species Crossreactivity: bovine. Does not react with rat, mouse, hamster, canine or rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Beisiegel, U., et al., J. Biol. Chem. 256: 11,923-11,931 (1981). 2. Beisiegel, U., et al., J. Biol. Chem. 257: 13,150-13,156 (1982). 3. Schmitz, G., et al., Arteriosclerosis & Thrombosis 13: 1053-1065 (1993). 4. Virgolini, I., et al., Arteriosclerosis, Thrombosis & Vascular Biology 15: 17-26 (1995).