Technical Data
L2601-02A
Lipoprotein Receptor, Low Density (LDLR, LDL Receptor) (Biotin)
Description:
The LDL receptor (160kD mature or glycosylated receptor; 120kD precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.

Application:
Suitable for use in ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:20
Optimal dilutions to be determined by the researcher.

Positive Control:
HepG2 cells and liver sections

Negative Control:
NIH 3T3 cells and normal skin

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k4C5Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified bovine adrenal cortex LDL Receptor
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.5% BSA. Labeled with Biotin.
Specificity:
Recognizes an epitope in the region of repeat #1 of the human ligand binding region. Species Crossreactivity: bovine. Does not react with rat, mouse, hamster, canine or rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beisiegel, U., et al., J. Biol. Chem. 256: 11,923-11,931 (1981). 2. Beisiegel, U., et al., J. Biol. Chem. 257: 13,150-13,156 (1982). 3. Schmitz, G., et al., Arteriosclerosis & Thrombosis 13: 1053-1065 (1993). 4. Virgolini, I., et al., Arteriosclerosis, Thrombosis & Vascular Biology 15: 17-26 (1995).