Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Receptor Binding Studies.
Flow Cytometry: 1:10
Western Blot: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not determined|
|Synthetic peptide of the LDLR extracellular domain (epitope between residues 184-195, the linker region between repeats 4 and 5)|
|Purified by immunoaffintiy chromatography|
|Supplied as a liquid in PBS, pH 7.4, 0.5% BSA|
|Recognizes the human LDL receptor. In immunoblotting, the antibody recognizes the 160 kD band of LDLR and a 120 kD band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum. Does not inhibit binding of LDL.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|