Technical Data
L2601-10
Lipoprotein, Low Density (LDL)
Description:
Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Malonyldialdehyde-treated LDL appear to be different from LDL oxidized by other means.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:20000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k4C7Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Low-density lipoprotein purified from human plasma and treated with malonyldialdehyde (MDA).
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity:
Recognizes MDA treated low-density lipoprotein (LDL, MDA-treated).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Palinski W, Yla-Herttuala S, Rosenfeld ME, Butler SW, Socher SA, Parthasarathy S, Curtiss LK, Witztum JL (1990) Antisera and monoclonal antibodies specific for epitopes generated during oxidative modification of low density lipoprotein. Arteriosclerosis 10:325-335.
2. Vaarala O, Alfthan G, Jauhiainen M, Leirisalo-Repo M, Aho K, Palosuo T (1993) Crossreaction between antibodies to oxidised low-density lipoprotein and to cardiolipin in systemic lupus erythematosus. Lancet 341:923-925.
3. Binder JC, Horkko S, Dewan A, Chang MK, Kieu EP, Goodyear CS, Shaw PX, Palinski W, Witztum JL, Silverman GJ (2003) Pneumococcal vaccination decreases atherosclerotic lesion formation: molecular mimicry between Streptococcus pneumoniae and oxidized LDL. Nat Med 9:736-743.