Technical Data
L2623-01A
LOX-1 (CLEC8A, hLOX-1, Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX1, LOX-1, oxidised low density lipoprotein (lectin-like) receptor 1, Oxidized low-density lipoprotein receptor 1, Ox-LDL rece
Description:
LOX-1 belongs to the C-type lectin-like protein superfamily. This unique scavenger receptor plays important roles in atherogenesis. Like other scavenger receptors, LOX-1 has a wide spectrum of physiological ligands, including OxLDL, anionic phospholipids, aged/apoptotic cells, activated platelets, and bacteria. The LOX-1 gene is a so-called immediate early gene that is dynamically modulated by several factors in vitro and in vivo. LOX-1 expression is induced by stimuli such as inflammatory cytokines, OxLDL, TNF-alpha, TGF-beta, and ANG II in vitro, and several proatherogenic factors in vivo. L2623-01A neutralizes LOX-1 and inhibits Hsp70 binding to dendritic cells and Hsp70-induced antigen cross-presentation. In vivo, targetting LOX-1 with a tumor antigen using anti-LOX-1 antibody induces antitumor immunity.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Neutralization and functional studies. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50. 10ug/ml was used on human peripheral blood myeloid DC and macrophages.
Fuctional studies: totally prevents Hsp70 binding to LOX-1-CHO but not mock-transfected CHO cells (1).
Optimal dilutions to be determined by the researcher.

Positive Control:
Human APCs

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19G219Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Fusion protein corresponding to the extracellular domain, aa71-273, of human LOX-1 with mouse Fcy1 (LOX-1-muFc) produced in PEAK cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Species crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Delneste, Y et al; Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation. Immunity 2002, 17: 353 2. Jeannin, P et al; Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors. Immunity 2005, 22: 551 3. Parlato, S et al; LOX-1 as a natural IFN- -mediated signal for apoptotic cell uptake and antigen presentation in edndritic cells. Blood 2010, 115: 1554