Technical Data
L2623-10A
Lipoxygenase, 5-, phosphorylated (Ser523) (5-LOX, 5-LO, 5-LPG, Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygenase, ALOX5, Leukotriene A4 Synthase, LOG5, MGC163204)
Description:
The enzyme 5-lipoxygenase (5-LO) play a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contribute to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994) fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis, and atherosclerosis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Phosphopeptide corresponding to amino acids residues surrounding the phospho Ser523 of human 5-Lipoxgenase (5-LO).
Purity:
Purified by immunoaffinity chromatography.
Form
10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol
Specificity:
Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots of rat hippocampal lysate. Species Crossreactivity: Recognizes human and rat forms of 5-LO.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Drazen JM, Lilly CM, Sperling R, Rubin P, Israel E (1994) Role of cysteinyl leukotrienes in spontaneous asthmatic
responses. Adv. Prostaglandin Thromboxane Leukot Res 22:251-262.

2. Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M (2004) Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. New England J Med 350:29-37.

3. Funk, CD (2001) Prostaglandins and leukotrienes: advances in eicosanoid biology. Science 294:1871-1875.

4. Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG (2004) Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on Serine 523. J Biol Chem 279:41,512-41,520.

5. Wilborn J, Bailie M, Coffey M, Burdick M, Strieter R, Peters-Golden M. (1996) Constitutive activation of 5-lipoxygenase in the lungs of patients with idiopathic pulmonary fibrosis. J Clin Invest 97:1827-1836.