		Technical Data
L2623-11A	Lysyl Oxidase (LOX, Protein-lysine 6-oxidase)

Description:
Protein-lysine-6 oxidase; LOX (EC 1.4.3.13), a 30kD copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of ECM. Localized both in the cytosol and nucleus, it catalyzes the oxidation and deamination of epsilon-NH2 group of lysine and hydroxylysine residues to alpha-aminoadipic-delta-semialdehyde. This is the first step that initiates the covalent cross-linking of collagen and tropoelastin resulting in the formation of insoluble collagen and elastic fibers in the extracellular matrix. LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. Deficiency of LOX is seen in certain carcinomas and 3X-linked recessive human connective tissue disorders—Cutis Laxa.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Immunohistochemistry (formalin fixed paraffin embedded): 1:100
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined.

Immunogen:

Synthetic peptide corresponding to aa305-320 (DLLDANTQRRVAEGHK) of human LOX.

Purity:

Serum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

Specificity:

Recognizes human LOX. Species Crossreactivity: chimpanzee, bovine, mouse, rat. Species sequence homology: chicken, canine, porcine, xenopus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Mariani,T.J., Trackman,P.C., Kagan,H.M., Eddy,R.L., Shows,T.B., Boyd,C.D. and Deak,S.B. The complete derived amino acid sequence of human lysyl oxidase and assignment of the gene to chromosome 5 (extensive sequence homology with the murine ras recision gene). Matrix 12 (3), 242-248 (1992). 2. Kaneda,A., Wakazono,K., Tsukamoto,T., Watanabe,N., Yagi,Y., Tatematsu,M., Kaminishi,M., Sugimura,T. and Ushijima,T. Lysyl oxidase is a tumor suppressor gene inactivated by methylation and loss of heterozygosity in human gastric cancers. Cancer Res. 64 (18), 6410-6415 (2004). 3. Hong,H.H., Pischon,N., Santana,R.B., Palamakumbura,A.H., Chase,H.B., Gantz,D., Guo,Y., Uzel,M.I., Ma,D. and Trackman,P.C. A role for lysyl oxidase regulation in the control of normal collagen deposition in differentiating osteoblast cultures. J. Cell. Physiol. 200 (1), 53-62 (2004). 4. Fogelgren,B., Polgar,N., Szauter,K.M., Ujfaludi,Z., Laczko,R., Fong,K.S. and Csiszar,K. Cellular fibronectin binds to lysyl oxidase with high affinity and is critical for its proteolytic activation. J. Biol. Chem. 280 (26), 24690-24697 (2005).