Technical Data
L3220-50
LNK (Lymphocyte Adapter Protein, Lymphocyte Specific Adapter Protein Lnk, SH2B Adaptor Protein 3, SH2B3, Signal Transduction Protein Lnk)
Description:
T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM]

Positive Control: Tcell line MOLT4 lysate

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Corresponding to amino acids NGPALQPSSPC, from the N Terminus of the LNK protein sequence according to NP_005466.
Purity:
Purified by immunoaffinity chromatography.
Form
Tris saline containing 0.02% Na azide [pH7.3] with 0.5% BSA
Specificity:
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Li Y, He X, Schembri-King J, Jakes S, Hayashi J. Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation. J Immunol. 2000 May 15;164(10):5199-206. PMID: 10799879