Technical Data
L3421-15
LOPP (Lysyl Oxidase Pro-peptide)
Description:
Lysyl oxidase (LOX) is an extracellular enzyme that is critical for the normal biosynthesis of collagens and elastin. LOX is synthesized as a 50kD precursor, secreted into the extracellular matrix and subsequently proteolytically processed to a 30kD active enzyme and an 18kD propeptide. LOX also reverts ras-mediated transformation and its expression is down-regulated in human cancers. Most recently, the lysyl oxidase pro-peptide (LOPP) has been associated with tumor suppression, as it inhibits ras-dependent cellular transformation.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
MC3T3-E1 lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide derived from an internal region of the mouse protein (residues 78-115).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the mouse pro-enzyme (50kD) and pro-peptide. The pro-peptide runs at ~18-20kD when not glycosylated and ~35kD on SDS-PAGE when N-glycosylated. Species Crossreactivity: human, rat, primate and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Palamakumbura, AH., et al. The propeptide domain of lysyl oxidase induces phenotypic reversion of ras-transformed cells. JBC. 279(39): 40593-40600 (2004)