Technical Data
L3514-50B
LOX (LYOX, Lysyl Oxidase, MGC105112, Protein-Lysine 6-Oxidase)
Description:
Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extracellular matrix. In addition, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility, and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000. A band is seen at ~58kD. May see bands at ~32kD (mature, secreted form), 50kD and 58kD (pro and glycosylated forms) since L3514-50B is a highly glycosylated protein.
Immunohistochemistry (Paraffin): 1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Human kidney lysate, mouse kidney lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabPurified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~15mg/ml
Immunogen:
Two synthetic peptides corresponding to aa200-300 from human LOX and aa300-400 also from human LOX. Cellular Localization: Cytosolic, nuclear. Species Sequence Homology (92%): Chicken, zebrafish, xenopus.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.02% sodium azide.
Specificity:
Recognizes human LOX. Species Crossreactivity: Mouse, rat, bovine, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Trackman, PC., et al. Post-translational glycosylation and proteolytic processing of a lysyl oxidase precursor. J. Cell Biol. 267(12): 8666-8671 (1992).
2. Erler, JT., et al. Lysyl oxidase is essential for hypoxia-induced metastasis. Nature. 440(27): 1222-1226 (2006).
3. Thomassin, L., et al. The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers. J. Biol. Chem. 280(52): 42848-55 (2005).