		Technical Data
L3514-54	LOX1 (LOX-1, hLOX-1, C-type Lectin Domain Family 8 Member A, CLEC8A, Lectin-like Oxidized LDL Receptor 1, Lectin-like oxLDL Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOXIN, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL Receptor 1, OLR1, SCARE

Description:
Type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1) is a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10; 10ul labels 10e6 cells in 100ul.
Immunoprecipitation: Neat
Western Blot: 1:10-1:100. Detects intact 50kD LOX-1 and also a prominent 32kD proteolytic fragmant.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Sheep

Concentration:

~0.1mg/ml

Immunogen:

Recombinant human extracellular domain LOX-1 protein from bacteria.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes human LOX-1 on transfected cells and in recombinant protein reconstitution assays.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Chen, M. et al. (2002) LOX-1, the receptor for oxidized low-density lipoprotein identified from endothelial cells: implications in endothelial dysfunction and atherosclerosis. Pharmacol. Ther. 95: 89-100. 2. Li, D. et al. (2003) LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells. Atherosclerosis, Thrombosis and Vascular Biology 23: 816-821. 3. Morawietz, H. et al. (1999) Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein. Circulation 100: 899-902. 4. Sawamura, T. et al. (1997) An endothelial receptor for oxidized low-density lipoprotein. Nature 386: 73-77. 5. Yoshida, H. et al. (1998) Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor. Biochem. J. 334: 9-13. 6. Murphy J.E., et al., Biochem J 393: 107-15 (2006)