Technical Data
L3514-54A
LOX1 (LOX-1, hLOX-1, C-type Lectin Domain Family 8 Member A, CLEC8A, Lectin-like Oxidized LDL Receptor 1, Lectin-like oxLDL Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOXIN, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL Receptor 1, OLR1, SCARE
Description:
LOX-1 is a receptor for oxidized low-density lipoproteins (LDL). This receptor mediates the recognition, internalization and degradation of oxidized LDL. It is a Type II membrane protein with a typical C-type lectin structure at the extracellular C-terminus which recognizes the ligand. LOX-1 activation by oxidized LDL causes endothelial changes such as decreased nitric oxide release and an increased expression of adhesion molecules. LOX-1 also binds activated platelets and apoptotic cells. The expression of LOX-1 is induced by pro-atherogenic conditions such as hyperlipidemia, hypertension and diabetes and as such appears to contribute to the pathogenesis of vascular disorders, particularly atherosclerosis.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:100-1:200. Fixation with 4% paraformaldehyde or methanol is recommended.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1903-30H: IgG, H&L, X-adsorbed (FITC) Pab Sh xRb
I1903-30T: IgG, H&L, X-adsorbed (Biotin) Pab Sh xRb
I1903-30U: IgG, H&L, X-adsorbed (HRP) Pab Sh xRb

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Peptide corresponding to the C-terminal (extracellular) region of human LOX-1.
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes the extracellular domain of human LOX-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen, M. et al. (2002) LOX-1, the receptor for oxidized low-density lipoprotein identified from endothelial cells: implications in endothelial dysfunction and atherosclerosis. Pharmacol. Ther. 95: 89-100. 2. Li, D. et al. (2003) LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells. Atherosclerosis, Thrombosis and Vascular Biology 23: 816-821. 3. Morawietz, H. et al. (1999) Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein. Circulation 100: 899-902. 4. Sawamura, T. et al. (1997) An endothelial receptor for oxidized low-density lipoprotein. Nature 386: 73-77. 5. Yoshida, H. et al. (1998) Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor. Biochem. J. 334: 9-13.