Technical Data
L4500-03
LRP, MVP (Major Vault Protein, Lung-resistance Related Protein)
Description:
Application(s):
Immunohistochemistry (frozen sections, paraffin sections): use approx. 1:20-50 dilution for staining of paraformaldehyde (4%) fixed cytospin preparations or frozen tissue sections. Apply the following pretreatment: 10 min in 20mM glycine (pH 7.5) and 10 min in 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5 (Schroeijers et al., 2000)
Western Blot: use 1:50 dilution of MVP-37 (ECL)

TypeIsotypeCloneGrade
MabIgG2b6D535Supernatant
SizeStorageShippingSourceHost
125ug-20CBlue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
Human MVP/LRP (major vault protein/lung resistance protein).
Purity:
Supernatant
Form
Liquid. Antibody in serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
Specificity:
Recognizes an internal epitope of human MVP/LRP, which is strongly overexpressed in various non-P-glycoprotein MDR tumor cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000)
2. Detection of the Mr 110,000 lung resistance-related protein LRP/MVP with monoclonal antibodies: A.B. Schroeijers, et al.; J. Histochem. Cytochem. 49, 1379 (2001)
3. Up-regulation of drug resistance-related vaults during dendritic cell development: A.B. Schroeijers, et al.; J. Immunol. 168, 1572 (2002)