Description:
Application(s):
Immunohistochemistry (frozen sections, paraffin sections): use approx. 1:20-50 dilution for staining of paraformaldehyde (4%) fixed cytospin preparations or frozen tissue sections. Apply the following pretreatment: 10 min in 20mM glycine (pH 7.5) and 10 min in 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5 (Schroeijers et al., 2000)
Western Blot: use 1:50 dilution of MVP-37 (ECL)
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 6D535 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 125ug | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~0.25mg/ml |
| Immunogen: |
| Human MVP/LRP (major vault protein/lung resistance protein). |
| Purity: |
| Supernatant |
| Form |
| Liquid. Antibody in serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
| Specificity: |
| Recognizes an internal epitope of human MVP/LRP, which is strongly overexpressed in various non-P-glycoprotein MDR tumor cell lines. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000)
2. Detection of the Mr 110,000 lung resistance-related protein LRP/MVP with monoclonal antibodies: A.B. Schroeijers, et al.; J. Histochem. Cytochem. 49, 1379 (2001)
3. Up-regulation of drug resistance-related vaults during dendritic cell development: A.B. Schroeijers, et al.; J. Immunol. 168, 1572 (2002)
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