Technical Data
L4502-20
LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin)
Description:
LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 1:100
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:500
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant Human LRRK2 (leucine-rich repeat kinase 2) (aa1838-2133) is fused at the N-terminus to a tag.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human LRRK2. The strongest recognized epitope is PDQPRLTIPISQ (aa1843-1854) by mapping.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. C. Paisan-Ruiz, et al. (2004) Neuron 44:595. 2. A.B. West, et al. (2005) PNAS 102:16842. 3. E. Meylan and J. Tschopp (2005) TIBS 30:151.