Technical Data
L5999-27
Luciferase
Description:
The antibody is derived from luciferase of the North American firefly (Photinus pyralis). Luciferase is a 61kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation. When expressed in mammalian cells, luciferase molecules produce luminescence in direct proportion to their number in the presence of luciferin and ATP. In this respect, Luciferase is commonly used as a promoter to quantify expression levels from promoters in tissue culture and in vivo.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein.
Immunohistochemistry (Frozen): 15-50ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers.
Immunofluorescence: 10-30ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A106Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
0.5mg/ml
Immunogen:
Luciferase protein from Photinus pyralis
Purity:
Purified by Protein A chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Specificity:
Recognizes luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tatsumi H. etal. (1996) Anal Biochem 243(1):176-80. 2. Van Leeuwen W. et al. (2000) Plant Mol. Biol. Reporter 18(2):143. 3. Chem B. et al. (1994) J. Virol 68(2): 54-60. 4. Kobatake E. et al. (1993) Anal Biochem 208(2):300-5. 5. Park S. et al. (1992) J. Gen Microbiol 138(12):2619-27. 6. Nicolas M. et al. (1989) 37(5):663-74.