		Technical Data
L5999-27	Luciferase

Description:
The antibody is derived from luciferase of the North American firefly (Photinus pyralis). Luciferase is a 61kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation. When expressed in mammalian cells, luciferase molecules produce luminescence in direct proportion to their number in the presence of luciferin and ATP. In this respect, Luciferase is commonly used as a promoter to quantify expression levels from promoters in tissue culture and in vivo.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein.
Immunohistochemistry (Frozen): 15-50ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers.
Immunofluorescence: 10-30ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6A106	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

0.5mg/ml

Immunogen:

Luciferase protein from Photinus pyralis

Purity:

Purified by Protein A chromatography

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

Specificity:

Recognizes luciferase. No cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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