Technical Data
L7715-34E
Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465) (PE)
Description:
Ly-6C is a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10D386Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
Not determined
Immunogen:
Balb/c macrophage precursor cell hybrids
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes mouse Ly-6C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur. J. Immunol. 20: 2734. 2. de Bruijn, M.F et al. (1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting. J. Immunol. Methods. 217: 27-39.