Technical Data
L7715-34G
Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465)
Description:
The antigen is a 14kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.


Applications:
Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen, paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aER-MP20Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells.
Purity:
Purified
Form
Supplied as a liquid in 0.02% sodium azide, 0.1% BSA.
Specificity:
Recognizes specifically mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. de Bruijn, M et al; Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens. Eur J Immunol 1994, 24: 2279 2. de Bruijn, M et al; Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting. J Immunol Methods 1998, 217: 27 3. Chan, J et al; Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression. Blood 1998, 92: 1423
4. Henkel, G et al; Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1. Blood 1999, 93: 2849 5. Manitz, M et al; Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro. Mol Cell Biol 2003, 23: 1034.