Technical Data
L7715-35
Ly-6C (Lymphocyte Antigen 6 Complex, Locus C)
Description:
Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.1-4

Applications:
Suitable for use in Flow Cytometry and In vitro activation of T-lymphocytes. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2c,kHK1.4Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
L3 cloned CTL cells
Purity:
Purified
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Specificity:
Recognizes mouse Ly-6C, Mr 18kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Herold, K.C., A.G. Montag, S.M. Meyer, C. Wojcikowski, and F.W. Fitch. 1990. Diabetes 39:815. 2. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, S. Fleming, L.A. Herzenberg, E.L. Berg, and E.C. Butcher. 1988. Eur. J. Immunol. 18:1819. 3. Johnson, R., D.W. Lancki, and F.W. Fitch. 1993. J. Immunol. 151:2986. 4. Lancki, D.W., P. Fields, D. Qian, and F.W. Fitch. 1995. Immunol. Rev. 146:117.