		Technical Data
L7715-35C	Ly-6C (Lymphocyte Antigen 6 Complex, Locus C) (PE)

Description:
Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.1-4

Applications:
Suitable for use in Flow cytometry and
In vitro activation of T lymphocytes. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

Type	Isotype	Clone	Grade
Mab	IgG2c,k	HK1.4	Purified

Size		Storage	Shipping	Source	Host
100ug		4°C Do not freeze	Blue Ice	Mouse	Rat

Concentration:

~0.1mg/ml

Immunogen:

L3 cloned CTL cells

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.

Specificity:

Recognizes murine Ly-6C, Mr 18kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Herold, K.C., A.G. Montag, S.M. Meyer, C. Wojcikowski, and F.W. Fitch. 1990. Diabetes 39:815.2. Jutila, M.A., F.G.M. Kroese, K.L. Jutila, A.M. Stall, S. Fleming, L.A. Herzenberg, E.L. Berg, and E.C. Butcher. 1988. Eur. J. Immunol. 18:1819.3. Johnson, R., D.W. Lancki, and F.W. Fitch. 1993. J. Immunol. 151:2986.4. Lancki, D.W., P. Fields, D. Qian, and F.W. Fitch. 1995. Immunol. Rev. 146:117.