Technical Data
Lyme Disease, Human, IgG, BioAssay™ ELISA Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
The United States Biological Lyme Disease, Human, IgG, BioAssay™ ELISA Kit is designed to detect IgG class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen.

Kit Components:
L8001-20A: Microtiter Plate: 1x96wells
L8001-20B: IgG, Goat x human (HRP): 1x15ml
L8001-20C: Positive Control: 1x350ul
L8001-20D: Calibrator: 1x500ul
L8001-20E: Negative Control: 1x350ul
L8001-20F: Sample Diluent: 1x30ml
L8001-20G: Tetramethylbenzidine (TMB): 1x15ml
L8001-20H: Stop Solution:1x15ml (1N H2SO4, 0.7N HCl)
L8001-20J: Wash Buffer, 10X: 1x100ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Steere, A.C., Taylor, E., Wilson, M.L., Levine, J.F., and Spielman, A.: Longitudinal assessment of the clinical and epidemiologic features of Lyme disease in a defined population. J. Infect. Dis. 154: 295-300 (1986). 2. Rosenfeld, M.E.A.: Serodiagnosis of Lyme disease. J. Clin. Microbiol. 31: 3090-3095 (1993). 3. Steere, A.C., et al., New Engl. J. Med. 308: 733 (1983). 4. Bakken, L.L., et al., J. Clin. Microbiol. 35: 537-543 (1997). 5. U.S. Department of Labor, Occupational Safety and Health Administration: Occupational Exposure to Bloodborne Pathogens. Final Rule. Fed. Register 56: 64175-64182 (1991). 6. Procedures for the collection of diagnostic blood specimens by venipuncture - Second edition: Approved Standard (1984). Published by National Committee for Clinical Laboratory Standards. 7. Procedures for the Handling and Processing of Blood Specimens. NCCLS Document H18-A, Vol. 10, No. 12, Approved Guideline (1990). 8. Russel, H., Sampson, J.S., Schmid, G.P., Wilkinson, H.W., and Plikaytis, B.: Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J. Infect. Dis. 149: 465 (1984). 9. Magnarelli, L.A., et al., J. Infect. Dis. 156: 183-188 (1987). 10. Hunter, E.F., et al., Sex. Trans. Dis. 3: 236 (1986). 11. Steere, A.C., et al., Ann. Intern. Med. 99: 22 (1983). 12. Craft, J.E., et al., Yale J. Biol. Med. 57: 561 (1984). 13. Shrestha, M., et al., Am. J. Med. 78: 235 (1985). 14. Reik, L., et al., Neurology 35: 267 (1985). 15. Barbour, A.:Laboratory Aspects of Lyme Borreliosis. Clin Micr. Rev. 1: 399-414 (1988).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.