Technical Data
L9058-06
Lysine, acetylated, N Epsilon (Acetyl-Lysine)
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Technical Data |
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L9058-06 |
Lysine, acetylated, N Epsilon (Acetyl-Lysine) |
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Description: Nucleosome composition varies considerably from one chromosomal domain to the next, with a major variable being histone acetylation. The level of histone acetylation also correlates directly with the transcriptional activity of a particular domain. Histone acetylation destabilizes the nucleosome structure and renders the DNA more accessible to transcription factors. This destabilization is the result of the reduction in the capacity of the acetylated N-terminal tails to stabilize the path of DNA in the nucleosome through charge neutralization. Recent studies of the several enzymes involved in acetylation and deacetylation of histone residues have revealed a possible relationship between gene transcriptional activation, histone acetylation and tumor suppressor function. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 1:5000-1:50,000 Immunohistochemistry: 1:500-1:5000 Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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