Technical Data
L9190-09C
Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA) (PE)
Description:
CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul/10e6 cells
Immunohistochemistry: acetone-fixed, frozen tissue sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG15K76Purified
SizeStorageShippingSourceHost
100Tests4C Do not freezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human Lysosome Associated Membrane Protein 1
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human LAMP 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: Cell Differentiation Antigens, Oxford University Press, Oxford2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD107a, 107b Section, Academic Press, New York, p. 3893. Sawada, R., K.A. Jardine, and M. Fukuda. 1993. J. Biol. Chem. 268:90144. Febbraio, M., and R.L. Silverstein. 1990. J. Biol. Chem. 265:185315. Chen, J.W., Y. Cha, K.U. Yuksel, R.W. Gracy, and J.T. August. 1988. J. Biol. Chem. 263:87546. Sawada, R., J.B. Lowe, and M. Fukuda. 1993. J. Biol. Chem. 268:12675 7. Sawada, R., S. Tsuboi, and M. Fukuda. 1994. J. Biol. Chem. 269:1425.