		Technical Data
M1011-50	M13, fd, F1 Bacteriophages, Filamentous

Description:
Applications:
Suitable for use in ELISA, Immunoblot and Immunoelectron microscopy. Also suitable for use in Phage Display (immunoassays for the identification of recombinant antigen- or antibody-phages). Other applications not tested.

Recommended Dilution:
ELISA: 1:500
Phage Display: Detection limit: 10e7 phage particles
Optimal dilution to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	5F167	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	E. coli	Mouse

Concentration:

~0.05mg/ml

Immunogen:

fd phages from E. coli F+ strain (JM109).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to 1ml with sterile dH2O or 40-50% glycerol.

Specificity:

Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O: Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171, 103-109 (1994)
Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999)
Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotechnology 19, 75-78 (2001)