Technical Data
M1011-50A
M13, fd, F1 Bacteriophages, Filamentous
Description:
Applications:
Suitable for use in ELISA, Western Blot, Phage Display and Immunoelctron Microscopy. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Phage Display: 10e7 phage particles (Detection Limit).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Antibody shows a tendency to precipitate at neutral pH.
TypeIsotypeCloneGrade
MabIgG2b5F167Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceE. coliMouse
Concentration:
~0.1mg/ml (after reconstitution)
Immunogen:
fd phages from E. coli F+ strain (JM109).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p [AEGDDPAKAAFDSLQASAT].
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Micheel, B., et al., J. Immunol. Methods 171: 103-109 (1994). 2. Kneissel, S., et al., J. Mol. Biol. 288: 21-28 (1999). 3. Rondot, S., et al., Nature Biotechnology 19: 75-78 (2001).