		Technical Data
M1011-50A	M13, fd, F1 Bacteriophages, Filamentous

Description:
Applications:
Suitable for use in ELISA, Western Blot, Phage Display and Immunoelctron Microscopy. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Phage Display: 10e7 phage particles (Detection Limit).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Antibody shows a tendency to precipitate at neutral pH.

Type	Isotype	Clone	Grade
Mab	IgG2b	5F167	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	E. coli	Mouse

Concentration:

~0.1mg/ml (after reconstitution)

Immunogen:

fd phages from E. coli F+ strain (JM109).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

Specificity:

Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p [AEGDDPAKAAFDSLQASAT].

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Micheel, B., et al., J. Immunol. Methods 171: 103-109 (1994). 2. Kneissel, S., et al., J. Mol. Biol. 288: 21-28 (1999). 3. Rondot, S., et al., Nature Biotechnology 19: 75-78 (2001).