Technical Data
M1011-50C
M13, fd, F1 Bacteriophages, Filamentous (Biotin)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Phage Display
(immunoassays for the identification of recombinant antigen- or antibody-phages; detection limit: 10e7 phage particles) Other applications not tested..

Recommended Dilution:
ELISA: 1ug/ml (dilution 1:500-1:1000)
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
TypeIsotypeCloneGrade
MabIgG2b5F167Affinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceE. coliMouse
Concentration:
~0.4mg/ml
Immunogen:
fd phages from E. coli F+ strain (JM109).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Labeled with Biotin.
Specificity:
Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171,103-109 (1994). 2. Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999). 3. Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotechnology 19, 75-78 (2001).