Technical Data
M1011-50E
M13, fd, F1 Bacteriophages, Filamentous (HRP)
Description:
Applications:
Suitable for use in ELISA. Also suitable for Phage Display (immunoassay for identification of recombinant antigen- or antibody-phages). Other applications not tested.

Recommended Dilutions:
ELISA: 1:20
Phage Display: The detection limit is 10e7 phage particles
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.áLabeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b5F167Affinity Purified
SizeStorageShippingSourceHost
750ul-20░CBlue IceE. coliMouse
Concentration:
Not Determined
Immunogen:
fd phages from E. coli F+ strain (JM109).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid. Labeled with Horseradish Peroxidase (HRP).
Specificity:
Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Micheel B, et al., (1994) J. Immunol. Methods 171:103-109. 2. Kneissel S, (1999) J. Mol. Biol. 288:21-28. 3. Rondot S, et al., (2001) Nature Biotechnology 19:75-78.