Technical Data
M1011-50F
M13, fd, F1 Bacteriophages, Filamentous (Biotin)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Phage Display. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:10000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9L390Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceE. coliMouse
Concentration:
Not determined
Immunogen:
fd phages from E. coli F+ strain (JM109)
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Micheel, B., et al., (1994), "Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages", J. Immunol. Methods, 171, 103-109, 2. Kneissel, S., et al., (1999), "Epitope structures recognizes by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae)", J. Mol. Biol., 288, 21-28., 3. Rondot, S., et al., (2001), "A helper phage to improve single-chain antibody presentation in pahge display", Nature Biotechnology, 19, 75-78.