		Technical Data
M1011-51	M13, fd, F1 Bacteriophages, Filamentous

Description:
Applications:
Suitable for use in ELISA, Immunoblot and Phage Display (immunoassays for the identification of recombinant antigen- or antibody-phages) (Detection limit: 10e6 phage particles).

Recommended Dilution:
ELISA: 1:10

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	5F167	Supernatant

Size		Storage	Shipping	Source	Host
5ml		-20°C	Blue Ice	E. coli	Mouse

Concentration:

Not determined

Immunogen:

fd phages from E. coli F+ strain (JM109).

Purity:

Hybridoma culture supernatant

Form

Supplied as a liquid.

Specificity:

Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171, 103-109 (1994)
Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999)
Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotech-nology 19, 75-78 (2001)