Technical Data

Media

MacConkey Agar (Powder)

M1100

Microbiological Grade

Storage RT/4°C Shipping RT

Formulation in grams per Liter:


Pancreatic Digest of Gelatin	17.0
Agar	13.5
Lactose	10.0
Sodium Chloride	5.0
Bile Salts	1.5
Pancreatic Digest of Casein	1.5
Peptic Digest of Animal Tissue	1.5
Neutral Red	0.03
Crystal Violet	0.0001

Total:	50g/Liter

MacConkey agar is a selective and differential medium for detection of coliforms and other enteric organisms.

Appearance:
Beige with pale pink tint, homogenous, free-flowing powder.

pH:
7.1 ± 0.2 at 25°C

Microbiology:
The medium was prepared according to formulation and inoculated with the organisms listed below. Cultures were incubated at 35±2°C in an aerobic atmosphere and examined for growth at 18-24 hours.

Enterococcus faecalis ATCC 29212:
Partial to complete inhibition

Escherichia coli ATCC 25922:
Growth

Proteus mirabilis ATCC 12453:
Growth, partial inhibition of swarming

Salmonella typhimurium
ATCC 14028:
Growth

Directions per Liter:
Dissolve 50 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Directions per Liter Dissolve 50 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.