Technical Data
Media
MacConkey Agar (Powder)
M1100
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation in grams per Liter:
Pancreatic Digest of Gelatin17.0
Agar13.5
Lactose10.0
Sodium Chloride5.0
Bile Salts1.5
Pancreatic Digest of Casein1.5
Peptic Digest of Animal Tissue1.5
Neutral Red0.03
Crystal Violet0.0001
Total:50g/Liter
MacConkey agar is a selective and differential medium for detection of coliforms and other enteric organisms.

Appearance:
Beige with pale pink tint, homogenous, free-flowing powder.

pH (5%, 25C):
7.1 0.2

Microbiology:
The medium was prepared according to formulation and inoculated with the organisms listed below. Cultures were incubated at 352C in an aerobic atmosphere and examined for growth at 18-24 hours.

Enterococcus faecalis ATCC 29212:
Partial to complete inhibition

Escherichia coli ATCC 25922:
Growth

Proteus mirabilis ATCC 12453:
Growth, partial inhibition of swarming

Salmonella typhimurium
ATCC 14028:
Growth

Directions per Liter:
Dissolve 50 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions per Liter Dissolve 50 grams per liter of distilled/deionized (DDI) water, heating with stirring boiling for one minute until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.