Technical Data
Media
MacConkey Agar w/o Crystal Violet w/o NaCl (Powder)
M1100-25
Microbiological Grade
Storage RT    Shipping RT
Formulation (g/liter)
Enzymatic Digest of Gelatin17.0
Agar12.0
Lactose10.0
Sodium ChlorideAbsent
Bile Salts Mixture5.0
Enzymatic Digest of Casein1.5
Enzymatic Digest of Animal Tissue1.5
Neutral Red0.05
Crystal VioletAbsent
Total47g/liter
Used for the isolation and differentiation of gram-negative enteric bacilli. Restricts swarming of most Proteus sp. used to detect enteric bacteria and gram positive bacteria from urine.

Microbiological Analysis:
Escherichia coli ATCC 25922 growth, bright pink colonies
Enterococcus faecalis ATCC 29212 partial growth, inhibition pink to red
Proteus mirabilis ATCC 12453 growth, part. inhib. of swarming colorless colonies
Salmonella typhimurium ATCC 14028 growth, colorless colonies
Staphylococcus aureus ATCC 25923 growth, pink



Directions per Liter Dissolve 47 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Final pH: 7.4 ± 0.2 at 25°C Appearance

Microbiological Analysis: Escherichia coli ATCC 25922 growth, bright pink colonies Enterococcus faecalis ATCC 29212 partial growth, inhibition pink to red Proteus mirabilis ATCC 12453 growth, part. inhib. of swarming colorless colonies Salmonella typhimurium ATCC 14028 growth, colorless colonies Staphylococcus aureus ATCC 25923 growth, pink Important Note


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.