Technical Data
M1199-75C
Macrophage (Macrophages)
Description:
Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen/ Paraffin): 1:10-1:100
Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b9L463Supernatant
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human spleen cell homogenate, depleted of lymphocytes and erythrocytes.
Purity:
Supernatant
Form
Supplied as a liquid, 0.7% BSA, 0.09% sodium azide.
Specificity:
Recognizes an antigen expressed by human macrophages, monocytes and histiocytes. Does not cross-react with interdigitating dendritic cells, Langerhans cells, microglial cells or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen. In immunohistochemical studies the antibody shows partial cross-blocking with some anti-CD68 monoclonal antibodies.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jaspars, E.H., et al., (1994), "A new monoclonal antibody (3A5) that recognizes a fixative resistant epitope on tissue macrophages and monocytes", J. Clin. Pathol., 47: 248-252.