Technical Data
M1201-03B
Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
M-CSF is produced by a wide variety of cells including fibroblasts, monocytes, lymphocytes, osteoblasts, and mesenchymal cells to name a few. It stimulates the proliferation and differentiation of hematopoietic cells and plays an important role in the life of blood monocytes. Comprised of two 159 amino acid chains, this disulfide-linked homodimer has a molecular weight of 36.8kD.

Recombinant protein corresponding to mouse Macrophage Colony Stimulating Factor expressed in E. coli.

Biological Activity:
The activity is determined by the dose-dependent induction of M-NFS-60 cell proliferation and is typically 1.1-1.6ng/ml.

AA Sequence:
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
36.8kD
Source: E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.. Endotoxin: 0.1EU/ug
Form: Supplied as a lyophilized powder, pH 8.0. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.